1498 Identification and Distribution of F 9 Antigen Components
نویسنده
چکیده
T h e cell surface F9 ant igens are def ined by the 129/Sv an t i -F9 serum (1), a syngeneic an t i se rum raised agains t i r r ad ia t ed cells of the mur ine F9 e m b r y o n a l ca rc inoma (E.C.) 1 line (2). T h r o u g h c o m p l e m e n t m e d i a t e d cytotoxic i ty or indirect immunof luorescence (IF) tests, the F9 ant igens a p p e a r to be cell surface ant igens character is t ic of EC cells and thei r no rma l embryon ic coun te rpar t . T h e y are also found on cells of the male germ line (1, 3, 4). It has been shown recent ly (5) tha t the a n t i b o d y response of 129/Sv mice agains t F9 cells is complex; near ly all the cytotoxic ac t iv i ty of the serum is associated wi th the IgM ant ibodies , whereas the b in d ing of IgM, IgGl, a n d IgG2a ,b an t i -F9 an t ibodies to the surface of F9 cells can be revealed by IF. W e have isolated the an t i -F9 IgM, IgG1, and IgG2a ,b an t ibodies from the c rude ant i -F9 serum using an aff ini ty c h r o m a t o g r a p h y t echn ique (6). T h e activi t ies of the thus isolated Ig were tested by IF on a pane l of cell types. T h e results repor ted in the present p a p e r suggest tha t the an t i -F9 an t ibodies react wi th three i ndependen t ant igenic de te rminants .
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